Let us take one of the simplest cases--an alteration in form. Acylindrical cell of the alga Stigeoclonium assumes, as Livingstone (Livingstone, "On the nature of the stimulus which causes the change of form, etc." "Botanical Gazette", XXX. 1900; also XXXII. 1901.) has shown, a spherical form when the osmotic pressure of the culture fluid is increased; or a spore of Mucor, which, in a sugar solution grows into a branched filament, in the presence of a small quantity of acid (hydrogen ions) becomes a comparatively large sphere. (Ritter, "Ueber Kugelhefe, etc." "Ber. bot. Gesell." Berlin, XXV. page 255, 1907.) In both cases there has undoubtedly been an alteration in the osmotic pressure of the cell-sap, but this does not suffice to explain the alteration in form, since the unknown alterations, which are induced in the protoplasm, must in their turn influence the cell-membrane. In the case of the very much more complex alterations in form, such as we encounter in the course of development of plants, there do not appear to be any clues which lead us to a deeper insight into the phenomena. Nevertheless we continue the attempt, seeking with the help of any available hypothesis for points of attack, which may enable us to acquire a more complete mastery of physiological methods. To quote a single example; I may put the question, what internal changes produce a transition from vegetative growth to sexual reproduction?
The facts, which are as clearly established from the lower as for the higher plants, teach us that quantitative alteration in the environment produces such a transition. This suggests the conclusion that quantitative internal changes in the cells, and with them disturbances in the degree of concentration, are induced, through which the chemical reactions are led in the direction of sexual reproduction. An increase in the production of organic substances in the presence of light, chiefly of the carbohydrates, with a simultaneous decrease in the amount of inorganic salts and water, are the cause of the disturbance and at the same time of the alteration in the direction of development. Possibly indeed mineral salts as such are not in question, but only in the form of other organic combinations, particularly proteid material, so that we are concerned with an alteration in the relation of the carbohydrates and proteids. The difficulties of such researches are very great because the methods are not yet sufficiently exact to demonstrate the frequently small quantitative differences in chemical composition. Questions relating to the enzymes, which are of the greatest importance in all these life-processes, are especially complicated. In any case it is the necessary result of such an hypothesis that we must employ chemical methods of investigation in dealing with problems connected with the physiology of form.
II. INFLUENCE OF ENVIRONMENT ON THE TRANSFORMATION OF SPECIES.
The study of the physiology of form-development in a pure species has already yielded results and makes slow but sure progress. The physiology of the possibility of the transformation of one species into another is based, as yet, rather on pious hope than on accomplished fact. From the first it appeared to be hopeless to investigate physiologically the origin of Linnean species and at the same time that of the natural system, an aim which Darwin had before him in his enduring work. The historical sequence of events, of which an organism is the expression, can only be treated hypothetically with the help of facts supplied by comparative morphology, the history of development, geographical distribution, and palaeontology.
(See Lotsy, "Vorlesungen" (Jena, I. 1906, II. 1908), for summary of the facts.) A glance at the controversy which is going on today in regard to different hypotheses shows that the same material may lead different investigators to form entirely different opinions. Our ultimate aim is to find a solution of the problem as to the cause of the origin of species.
Indeed such attempts are now being made: they are justified by the fact that under cultivation new and permanent strains are produced; the fundamental importance of this was first grasped by Darwin. New points of view in regard to these lines of inquiry have been adopted by H. de Vries who has succeeded in obtaining from Oenothera Lamarckiana a number of constant "elementary" species. Even if it is demonstrated that he was simply dealing with the complex splitting up of a hybrid (Bateson, "Reports to the Evolution Committee of the Royal Society", London, 1902; cf. also Lotsy, "Vorlesungen", Vol. I. page 234.), the facts adduced in no sense lose their very great value.
We must look at the problem in its simplest form; we find it in every case where a new race differs essentially from the original type in a single character only; for example, in the colour of the flowers or in the petalody of the stamens (doubling of flowers). In this connection we must keep in view the fact that every visible character in a plant is the resultant of the cooperation of specific structure, with its various potentialities, and the influence of the environment. We know, that in a pure species all characters vary, that a blue-flowering Campanula or a red Sempervivum can be converted by experiment into white-flowering forms, that a transformation of stamens into petals may be caused by fungi or by the influence of changed conditions of nutrition, or that plants in dry and poor soil become dwarfed. But so far as the experiments justify a conclusion, it would appear that such alterations are not inherited by the offspring. Like all other variations they appear only so long as special conditions prevail in the surroundings.
